protein purification

it is a biotechnology topic, protin purification is the main topic
i want to be spocefic in monoclonal antibody purification by A protin chromotography method .
structure:
1- why it is purified
2- who is used it
3- A protin chromotography process
4- chalanges with this technology
4- market leaders and market innovators
5- standerd and legislation including FDA
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